IJRAP International Journal of Research in Ayurveda and Pharmacy 2277-4343 Moksha Publishing House DOI: 10.7897/2277-4343.1105151 FORMULATION AND EVALUATION OF POLYHERBAL ANTI-ACNE GEL Sneha Bharti Harini Chowdary Vadlamudi * 11 -0001 110 113 The main aim of the research was to formulate and evaluate polyherbal anti-acne gel. Four herbal extracts i.e., leaves of Azadirachta indica, leaves of Carica papaya, rhizomes of Curcuma longa and fruits of Vitis vinifera were used for the formulation of anti-acne gel. Total eight formulations were prepared by using these four herbal extracts and two polymers as gelling agents; out of which one is natural (Aloe vera) and the other is synthetic (Carbopol 934). The prepared gels were evaluated for physical appearance, pH, viscosity, and Spreadability. Anti-bacterial activity for eight formulations was carried out and compared with two commercial formulations by using three bacterial strains like Staphylococcus aureus, Pseudomonas aeruginosa, and Enterococcus species. The study was performed for eight days with the help of agar well diffusion method. Diameters of zone of inhibition were recorded. The prepared polyherbal anti-acne gel showed good pH, viscosity and Spreadability. F6, prepared using 1% of the four herbal extracts using Carbopol 934 as a gelling base demonstrated strong antibacterial activity against all three bacterial species, across all formulations. Therefore, prepared polyherbal gel (F6) could be effective in acne treatment. Azadirachta indica Anti-acne Anti-bacterial activity Staphylococcus aureus Polyherbal Spreadability.