IJRAP International Journal of Research in Ayurveda and Pharmacy 2277-4343 Moksha Publishing House 10.7897/2277-4343.140232 EVALUATION OF CYTOTOXIC AND ANTICANCER ACTIVITY OF PANEEYA KSHARA ON COLON CANCER CELL LINES Gururaja D * Sneha Kumbhar Sahana Acharya 11 -0001 12 17 Kshara is a unique medicinal preparation mentioned in Ayurveda. It is an alkali prepared by burning different medicinal plants. Kshara has cytotoxic activity. It can be used internally and externally. Kshara is used internally as medicine and is called Paneeya kshara. Paneeya kshara is mentioned in the context of Gulma roga. Gulma is a disease with the cardinal feature of a mass per abdomen. Malignant conditions of the colon can be correlated to Gulma. So, this study was planned to prepare the Paneeya kshara mentioned in Gulma disease and study its cytotoxic activity and anticancer activity on colon cancer cell lines. The HEK 293 normal cell line was used to study the cytotoxic activity, and colon cancer cell lines COLO 205, COLO320 and HCT-15 cell lines were used to study the anticancer activity. The cytotoxic activity on HEK293 normal cell lines was good, with an IC50 value of 0.726 __ampersandsignmicro;g. IC 50 value for COLO320 cell lines was 0.7263 __ampersandsignmicro;g. The effect on HCT -15 and COLO205 cell lines was insignificant, with an IC50 value of more than one. Paneeya kshara showed good cytotoxic action but limited anticancer activity on colon cancer cell lines. This study revealed the potentiality of Paneeya kshara in the management of colon cancer. Ayurveda Alkali preparation Colon cancer Gulma MTT assay Paneeya kshara