IJRAP International Journal of Research in Ayurveda and Pharmacy 2277-4343 Moksha Publishing House 10.7897/2277-4343.154115 IN VITRO PROLIFERATIVE EFFICACY OF AN INNOVATIVE NATURAL CALCIUM FORMULATION IN BONE AND MUSCLE CELLS Baidyanath Mishra * Dharmendra Kumar Ruchi Srivastava Varun Kumar Singh Gajender Apratim Sai Rajesh and Yathapu Srinivasa Reddy 11 -0001 47 49 Calcium is an essential macronutrient with a critical role in structural, functional and metabolic processes, particularly bone and muscle systems. Although dietary intake typically meets calcium needs, uneven food habits, anti-nutrients and various pathophysiological conditions can lead to deficiencies. Addressing this gap, Dabur India Limited developed an Innovative Natural Calcium Formulation (INCF) featuring natural organic calcium, plant-derived vitamin D3 and lemon juice. Two dosage forms providing 250 mg and 500 mg of calcium were developed and tested for efficacy using in vitro proliferation of Chondrocyte (C20A4 - Bone) and Muscle (L6 rat skeletal) cells at noncytotoxic concentrations. Results showed chondrocyte proliferation of 9.9% - 24.4% and skeletal muscle proliferation of 10.94% - 28.46% compared to untreated control. These findings indicate the potential of INCF as an effective calcium supplement. Calcium Deficiency Calcium Supplement Bone and Muscle Development.