IJRAP International Journal of Research in Ayurveda and Pharmacy 2277-4343 Moksha Publishing House 10.7897/2277-4343.154121 QUALITATIVE ANALYSIS OF MAHISHADRAVAKA WITH SPECIAL REFERENCE TO LCMS STUDY Anu Joy * Ambili KS Sandhya VR Smitha Francis 11 -0001 75 80 Introduction: Mahishadravaka, a renowned Ayurvedic proprietary medicine, has been historically used for its therapeutic properties. It is the arka preparation (distillation process) of Mahisha bone (bone of a buffalo), along with other herbal medicines. It is traditionally used for Vata vyadhies (diseases caused by Vata). Materials and Method: In this study, we conducted a comprehensive LCMS analysis and vitamin D3 and Calcium analyses of Mahishadravaka. Identification and characterisation of bioactive compounds were also aimed at. Result: Our findings reveal a diverse array of compounds with promising pharmacological activities, including Anti-inflammatory, antioxidant, anti-microbial, and anticancer properties. This article provides an overview of the identified compounds and their reported medicinal benefits, shedding light on the potential mechanisms underlying the therapeutic efficacy of Mahishadravaka. Conclusion: The LCMS of Mahishadravaka unveiled a rich array of bioactive compounds with significant pharmacological potential. The elucidation of the bioactive constituents paves the way for further research and development of novel therapeutic formulations based on Ayurvedic principles. These compounds encompass a broad spectrum of therapeutic activities, validating the traditional use of Mahishadravaka in Ayurvedic medicine. The findings from this study provide a scientific basis for the therapeutic efficacy of Mahishadravaka and underscore its potential for developing novel therapeutic interventions targeting various diseases. Mahishadravaka LCMS study Calcium Vit D3 Bioactive compounds