IJRAP International Journal of Research in Ayurveda and Pharmacy 2277-4343 Moksha Publishing House 10.7897/2277-4343.155155 NATURE__ampersandsign#39;S NECTAR: UNVEILING THE THERAPEUTIC POTENTIAL OF AN AYURVEDIC HERBAL BLEND IN DIABETES MANAGEMENT Prerona Boruah * Aishwarya Rathod Sejal Yadav Aditya Yadav Mala Parab Pramodkumar P Gupta Sanjay Ghosh Dastidar 11 -0001 56 61 Traditional medicines derived from medicinal plants are widely used across the globe. Studying the therapeutic properties of these plants, particularly for conditions such as diabetes, is essential for developing natural treatment options. This study investigates the antidiabetic potential of an Ayurvedic herbal mixture, focusing on glucose uptake, alpha-amylase inhibition, antioxidant activity, and cytotoxicity. Various assays were employed to evaluate the herbal blend’s efficacy at different concentrations. Glucose uptake was assessed using yeast cells and the HepG2 cell line, while the alpha-amylase inhibition assay measured the mixture’s impact on this enzyme. The antioxidant activity was examined through a radical scavenging assay, and cytotoxicity was evaluated on HepG2 cells. Additionally, phytochemical screening identified active compounds within the herbal concoction. Results demonstrated that the Ayurvedic mixture significantly enhanced glucose uptake in yeast cells and the HepG2 cell line and inhibited alpha-amylase activity. The herbal blend also showed strong antioxidant activity, as indicated by radical scavenging. The phytochemical analysis confirmed the presence of bioactive compounds with potential therapeutic benefits, and the cytotoxicity assay revealed no significant cytotoxic effects on HepG2 cells at the tested concentrations. In summary, the study suggests that this Ayurvedic herbal mixture has promising antidiabetic and antioxidant properties, making it a potential natural therapy for diabetes. However, further research, including in vivo studies and clinical trials, is required to validate its therapeutic efficacy and safety. Diabetes Mellitus Glucose Uptake Antioxidants ?-Amylase HepG2 cell line.