IJRAP International Journal of Research in Ayurveda and Pharmacy 2277-4343 Moksha Publishing House 10.7897/2277-4343.174141 MECHANISTIC IN VITRO ASSESSMENT OF GLUCOSE-LOWERING AND INSULIN-SENSITIZING ACTIVITIES OF JAMUN NEEM KARELA KWATH JUICE: A POLYHERBAL FORMULATION FOR SUPPORTING HEALTHY BLOOD SUGAR BALANCE Ruchi Srivastava Mohammad Amaan Khan * Yogisha S Arjun Multani 11 -0001 78 85 Diabetes mellitus is marked by chronic hyperglycemia due to defective insulin secretion or action. This study evaluated the in vitro glycemic control potential of Jamun Neem Karela Kwath Juice, a novel polyherbal liquid formulation manufactured by Multani Pharmaceuticals Ltd., comprising traditional herbs: Syzygium cumini, Momordica charantia and Azadirachta indica. Glycemic control potential was evaluated through __ampersandsignalpha;-amylase and __ampersandsignalpha;-glucosidase inhibition assays, using acarbose as the standard, alongside glucose uptake in differentiated L6 myotubes. Cytotoxicity was assessed via MTT assay, with IC?? values derived from nonlinear regression in GraphPad Prism. Jamun Neem Karela Kwath Juice exhibited dose-dependent enzyme inhibition. It achieved significant __ampersandsignalpha;-amylase inhibition (maximum % inhibition: 31.24%) and potent __ampersandsignalpha;-glucosidase inhibition (maximum % inhibition: 47.27%). In L6 myotubes, the formulation markedly boosted glucose uptake, peaking at 2% concentration with a 3.48-fold increase over basal levels. MTT assay indicated concentration-dependent cytotoxicity (IC??: 11.93% in L6 cells), confirming viability at lower doses suitable for functional assays. These results demonstrated Multani Jamun Neem Karela Kwath Juice’s insulin-sensitizing properties and potential to promote peripheral glucose uptake, positioning it as a promising therapeutic agent to support healthy blood sugar balance. ?-amylase inhibition ?-glucosidase inhibition Acarbose Doxorubicin Glucose uptake assay MTT assay