ISSN Online: 2229-3566
ISSN Print: 2277-4343
Article: EVALUATION OF PHYTOCHEMICAL AND ANTIMICROBIAL PROPERTIES OF PHYTOLACCA OCTANDRA
Article Category: Original Research articles
Author: Vithya Eswari. D *, Maleeka Begum. S.F, Ravikumar. K
Abstract: Pharmacological and antibacterial properties of Phytolacca octandra leaf extracts were investigated against significant bacterial pathogens in the present research. Phytolacca octandraleaf powder was extracted with water, acetone and petroleum ether separately using Soxhlet extraction protocol. The phytochemical screening of Phytolacca octandraextracts was investigated for the presence or absence of different phytoconstituents like alkaloid, tannin, flavonoid, phenol, steroid, saponin, glycoside, amino acid, terpenoid and carbohydrates. The antibacterial activity of the acetone extractswereevaluated against the test organisms by well diffusion method. Acetone extracts revealed the presence of different pharmacological compounds like alkaloid, tannin, flavonoid, phenol, steroid, saponin and glycoside. MICs of Phytolacca octandraextracts ranged from 3.13mg/ml to 12.5mg/ml against all the test organisms. Antibacterial activity of Phytolacca octandrashowed maximum inhibitory zones of 21mm, 18mm, 19mm, 19mm and 20mm against respective organisms for 25mg/ml of acetone extracts. Three types of phytochemical compounds (phenol, tannin and flavonoids) analyzed to identify the bioactive compounds from the column purified fractions using TLC. Green colour spots with Rf value of 0.78 was observed indicating the presence of phenol. The presence of tannin indicated by grey colour spots with Rf value of 0.83. And flavonoids showed orange colour spots when exposed to aluminum chloride reagents. The Rf value was calculated as 0.86. The obtained results corroborate the importance of ethno-pharmacological surveys in the selection of Phytolacca octandraplants for bioactivity screening against significant bacterial pathogens.
Keyword: Phytolacca octandra, Phytochemical compounds, Minimum inhibitory concentration, Antibacterial activity, Acetone extracts.