Article: AN IN-VITRO STUDY ON ANTI-MICROBIAL ACTION OF APARAJITHA DHUPA

Article Category: Original Research articles

DOI: 10.7897/2277-4343.100117

Pages: 71-74

Author: Nidhin Chandran *, Anusree Dileep, Jyothilal K

Abstract: Air contains millions of pathogenic and nonpathogenic microorganisms. Pathogenic microorganism is responsible for various health related problems.Fumigation can be applied as a preventive and curative therapy.Traditional fumigation technique using Aparajitha Dh?pa may serve the purpose of reducing the microbial load to non-pathogenic level. The aim of the study is to investigate the Anti-microbial action of Aparajitha dh?paagainst the Air born bacteria Streptococcus pyogenes.Aparajitha dh?pa includes eight drugs such as Pura(Commiphora mukul), Dhy?ma (Cymbopogon martini), Vac? (Acorus calamus), Sa?ja(Vateria indica), Nimba(Azadirachta indica), Arka(Calotropis procera), Agaru(Aquilaria agallocha) and Dh?ru(Cedrus deodara).A variety of laboratory methods can be used to evaluate or screen the in-vitroantimicrobial activity of an extract or a pure compound. Some of the most known and basic methods includes, Disc diffusion, Well diffusion, and Serial dilution methods. In this study, Serial dilution method is adopted to test the anti-microbial action of Aparajitha dh?pa. There was a marked reduction in the number of colonies in the test group compared to the control group of Streptococcus pyogenes. The test Petri dishes showed only 20 colonies of Streptococcus pyogenesin 10^-1dilution; whereas the control Petri dishes showed numerous colonies of Streptococcus pyogenesin 10^-1dilution in comparison to 10^-2dilution. The colony forming unit in 10^-1dilution and 10^-2dilution was too numerous to count. Colonies were greater than 300. Aparajitha dh?pashows an in-vitroantibacterial activity in the selected organism. There is a marked inhibition in the growth of Streptococcus pyogenes.

Keyword: Aparajitha dh?pa, Streptococcus pyogenes, Serial dilution