International Journal of Research in Ayurveda and Pharmacy

ISSN Online:2229-3566

ISSN Print: 2277-4343


Article Category: Original Research articles

DOI: 10.7897/2277-4343.100489

Pages: 68-75

Author: Nagaraj Ambanna Natikar, Mallappa Shalavadi *, Shanta Killer, Shravankumar Kolli, Chandrashekhar VM and Raghavendra HL

Abstract: The present article deals with study of pharmacognostic analysis of Curcuma vamana plant. In this, microscopic analysis of Curcuma vamana was done by transverse sections of rhizome, root, stem and leaf. The physicochemical evaluation; ash values, namely total ash, acid-insoluble ash, water-soluble ash, and sulfated ash; extractive values, namely alcohol soluble extractive value, water-soluble extractive and chloroform soluble extractive value; and loss on drying were determined. Preliminary phytochemical screening was done, and total phenolic content was estimated by Folin–Ciocalteu method and total flavonoids content was measured with the aluminum chloride colorimetric assay. In results, transverse section of Curcuma vamana rhizome, root, stem and leaf revealed that the presence most of microscopic features. Physicochemical screening of the rhizome powder showed 8.33% total ash, 1% acid insoluble ash, 9.66% water soluble ash, 8.66 sulphated ash, 8% water soluble extractive, 4.8% alcohol soluble extractive, 1.04% chloroform soluble extractive and 14% loss on drying. Preliminary phytochemical screening was shown presence of carbohydrates, proteins and amino acids, glycosides, alkaloids, phytosteroids, flavonoids, phenolic compounds, saponins and tannins. The total phenolic content in the ethanolic extracts were found 20.42 ± 0.4042 mg of gallic acid equivalent weight/g of extract and total flavonoids in plant ethanol extracts of Curcuma vamana were found 203.2 ± 1.749 mg of quercetin equivalent weight/g of extract. This information will be helpful in standardization for quality, purity and sample identification.

Keyword: Curcuma vamana, Ash values, Flavonoids, Phenols, Phytochemicals