Article: IN VITRO PHARMACOLOGICAL STUDY OF EXTRACT OF RAPHANUS SATIVUS SPROUTS

Article Category: Original Research articles

DOI: 10.7897/2277-4343.100492

Pages: 87-91

Author: P. Niharika *, K. Arathi, Lilly Grace, Y. Elisha, S. Manohar Babu

Abstract: Ethyl acetate extract of Raphanus sativus sprouts belonging to Brassicaceae, produced by cold maceration reveal the presence of volatile oils. Dilutions of the extract 20, 40, 60, 80, 100 µg/ml prepared in DMSO were used for the in vitro study. The antimicrobial activity of the extract was assessed using Bacillus subtilis (NCIM 2698), Staphylococcus aureus (NCIM 2079), Escherichia coli (NCIM 2344), Klebsiella pneumonia (NCIM 2957) and Aspergillus flavus (NCIM 568) against the standards gentamycin and ketoconazole. In vitro pharmacological studies, include the reducing activity, antioxidant activity and anti inflammatory activity were evaluated using the ferric reducing assay, hydrogen peroxide scavenging assay and albumin denaturation method respectively. The extract had shown significant antibacterial activity on all organisms excluding  Klebsiella and Escherichia than the standard. In vitro studies reveal significant reducing (36.015 + 0.686), antioxidant (26.830 + 0.240) and anti inflammatory activities (25.865 + 0.191) against the standard butylated hydroxyl toluene (BHT) (31.879 + 2.629), ascorbic acid (21.7245 + 0.240) and Diclofenac (0.4825 + 0.025) respectively.

Keyword: Raphanus sativus sprouts, anti microbial, reducing power, antioxidant activity, anti inflammatory activity.