Article: QUANTITATIVE DETERMINATION OF QUERCETIN A BIOMARKER IN METHANOLIC EXTRACT OF LAGERSTROEMIA LANCEOLATA AND LAGERSTROEMIA PARVIFLORA LEAVES BY HPTLC METHOD

Article Category: Original Research articles

DOI: 10.7897/2277-4343.1005108

Pages: 72-75

Author: Shubangi W. Jadhav *, R. B. Jadhav, Srinivas Rao

Abstract: In Indian Ayurvedic system, Lagerstroemia lanceolata (L. lanceolata) and Lagerstroemia parviflora (L. parviflora) are well-known plants used for major and minor ailments. Quercetin identified from the vast plethora of plant extracts has proved to possess ethno pharmacological relevance. The present investigation is to estimate biologically active flavonoid compound, quercetin in methanolic leaves extract of L. lanceolata and L. parviflora by using high-performance thin-layer chromatography (HPTLC). After extraction and phytochemical screening, the extracts were subjected to quantification for the presence of quercetin by HPTLC. Pre coated silica gel 60 F254 is used as a stationary phase and toluene: ethyl acetate: formic acid in ratio of 7: 5: 1 is used as a mobile phase. Densitometric estimation and quantification of quercetin was carried out at 254 nm. The standard Rf value of quercetin is 0.64. The total peak area of the standard, quercetin was compared and the corresponding peak areas of L. lanceolata and L. parviflora extracts were estimated to be 390.6 and 5442.8 respectively. A good linear relationship 0.988 was obtained between the concentration ranges of 0.2-1.0 µg. This HPTLC method was found to be simple and convenient for rapid screening of active compounds and quantification of the investigated flavonoids in L. lanceolata and L. parviflora.

Keyword: Lagerstroemia lanceolata, Lagerstroemia parviflora, HPTLC, Flavonoid compounds, Quercetin.