Article: ISOLATION AND PURIFICATION OF BIOACTIVE COMPOUNDS FROM THE MEDICINAL FERN ADIANTUM LATIFOLIUM LAM.

Article Category: Original Research articles

DOI: 10.7897/2277-4343.110116

Pages: 78-82

Author: Ramya Roselin I, S. Catharin Sara *, S. Sujatha, M. Gayathiri and R. Gnana Deepa Ruby

Abstract: The Pteridophytic plant Adiantum latifolium Lam. is an important medicinal fern belongs to the family Adiantaceae. The plant is popularly known as “broad leaf maidenhair fern” and they possess shiny black rachis, which is employed in folk medicine Worldwide. The plant is called “Avenca” in Brazil and famous for anti-inflammatory, anti- infectious and diuretic Properties. Adiantum latifolium Lam. has been used in Latin American traditional medicine as anxiolytic and analgesic. In the current investigation, Ethyl acetate extract of Adiantum latifolium Lam. was subjected to column chromatographic separation, which was successively eluted with Ethyl acetate, hexane, chloroform, methanol and their mixtures of increasing polarity. Elution with Chloroform: Methanol afforded a yellow color and this fraction was separated. The presence of Secondary metabolite Flavonoid was detected by Thin Layer chromatography, by recording Rf values. The isolated Phytoconstituent flavonoid was studied by Ultraviolet Visible Spectrophotometer and Fourier transform infrared spectroscopy. Ultraviolet Visible spectroscopy profile showed the peaks at 340 and 304 with the absorption of 0.072 and 10.061 respectively; which was taken at the wavelength of 100-1100 nm. The infrared spectrum confirmed the presence of Amines, Alkenes, Alkyl halide in the plant extract. Thus, the present experimental plant Adiantum latifolium Lam. possesses compounds with different curative properties and proved the beneficial role of bioactive compounds in human health is well documented. Further analysis for phytochemicals as well as therapeutic molecule present in this plant is continued.

Keyword: Adiantum latifolium Lam, Chromatography, Spectroscopy studies, secondary metabolites, phytoconstituent