Article: ASSESSMENT OF ANTIOXIDANT EFFECTS OF THE FORMULATION KUTAJA TRIPHALA: AN IN-VITRO STUDY

Article Category: Original Research articles

DOI: 10.7897/2277-4343.110356

Pages: 33-36

Author: Koottalakkal Sudhakaran Anu *, Krishnan Nirmala Anandalakshmi, Prabha Silpa, Thekkekkara Devassy Babu

Abstract: Ayurveda is one of the indigenous traditional systems of medicine for which scientific validation is useful for its more acceptances. Kutaja triphala (KT) mentioned in Panduroga Chikitsa of Ashtanga Hridaya. This formulation mainly used for the treatment of Pandu, kushta, jwara, pleeha, tamakaswasa, arsas, bhagandara, hritroga, gara, udara and prameha. The present study was aimed to evaluate the antioxidant activity of Kutaja triphala. Total Phenolic and flavonoid content of Kutaja triphala was determined with Folin – Ciocalteu and aluminum chloride method, respectively. The activity of Kutaja triphala on scavenging DPPH and hydroxyl radicals were analyzed. The reducing activity of Kutaja triphala was evaluated using FRAP (Ferric reducing antioxidant power) and PM (Phosphomolybdenum) assays. Kutaja triphala effectively scavenges DPPH and hydroxyl radicals with IC₅₀ value at 8.5 ± 2.17 and 44 ± 0.6 µg/mL respectively. The FRAP and PM assays had shown the dose dependent reducing activity of this drug. Kutaja triphala possess high Phenolic content (125.45 μg/mg GAE) and flavonoid content (72.25 μg/mg quercetin equivalent). In conclusion, the high Phenolic content and antioxidant property of this drug justify its uses in the treatment of diseases where oxidative stress plays a major role.

Keyword: antioxidants, Ayurveda, Kutaja triphala, free radicals, oxidative stress