Article: EVALUATION OF CYTOTOXIC AND ANTICANCER ACTIVITY OF PANEEYA KSHARA ON COLON CANCER CELL LINES

Article Category: Original Research articles

DOI: 10.7897/2277-4343.140232

Pages: 12-17

Author: Gururaja D *, Sneha Kumbhar, Sahana Acharya

Abstract: Kshara is a unique medicinal preparation mentioned in Ayurveda. It is an alkali prepared by burning different medicinal plants. Kshara has cytotoxic activity. It can be used internally and externally. Kshara is used internally as medicine and is called Paneeya kshara. Paneeya kshara is mentioned in the context of Gulma roga. Gulma is a disease with the cardinal feature of a mass per abdomen. Malignant conditions of the colon can be correlated to Gulma. So, this study was planned to prepare the Paneeya kshara mentioned in Gulma disease and study its cytotoxic activity and anticancer activity on colon cancer cell lines. The HEK 293 normal cell line was used to study the cytotoxic activity, and colon cancer cell lines COLO 205, COLO320 and HCT-15 cell lines were used to study the anticancer activity. The cytotoxic activity on HEK293 normal cell lines was good, with an IC₅₀ value of 0.726 µg. IC ₅₀ value for COLO320 cell lines was 0.7263 µg. The effect on HCT -15 and COLO205 cell lines was insignificant, with an IC₅₀ value of more than one. Paneeya kshara showed good cytotoxic action but limited anticancer activity on colon cancer cell lines. This study revealed the potentiality of Paneeya kshara in the management of colon cancer.

Keyword: Ayurveda Alkali preparation, Colon cancer, Gulma, MTT assay, Paneeya kshara