Article: BIOCHEMICAL AND CELLULAR CORRELATES OF ESSENTIAL OIL OF PISTACIA INTEGERRIMA J.L. STEWART EX BRANDIS GALLS AGAINST CISPLATIN-INDUCED NEPHROTOXICITY IN MICE

Article Category: Original Research articles

DOI: 10.7897/2277-4343.15384

Pages: 129-135

Author: Shirole RL *, Shirole NL, Suthariya BK, Saraf MN

Abstract: Ethnopharmacological relevance: Pistacia integerrima J.L. Stewart ex Brandis (Family Anacardiaceae) galls are used in Indian ethnomedicine for their antiasthmatic, antioxidant anti-inflammatory, sedative, and spasmolytic properties. However, no scientific studies demonstrate its nephroprotective potential against Cisplatin-induced nephrotoxicity. Materials and Methods: The effect of the essential oil of Pistacia integerrima (7.5, 15 and 30 mg/kg/day) and standard drug Vitamin E (250 mg/kg/day) on the kidney was examined 5 days after treatment with Cisplatin (12 mg/kg body weight, i.p.) induced oxidative renal damage in mice. Renal injury was assessed by assessment of serum creatinine and blood urea nitrogen. Renal oxidative stress was estimated by renal malondialdehyde levels, reduced glutathione levels and enzymatic activities of superoxide dismutase and catalase. Results: Essential oil of Pistacia integerrima (7.5, 15 and 30 mg/kg/day) significantly (p < 0.001) protected the nephrotoxicity induced by Cisplatin. The Cisplatin-induced increase in serum urea and creatinine concentrations was reduced in the essential oil of Pistacia integerrima treated groups. Essential oil of Pistacia integerrima treatment also increased the Cisplatin-induced decline of renal antioxidant enzymes such as superoxide dismutase, catalase, and Glutathione activities. Essential oil of Pistacia integerrima increased the concentration of reduced glutathione and protected the increase of Cisplatin-induced lipid peroxidation. Histopathological investigations of the kidney showed a protective effect of the essential oil of Pistacia integerrima in mice. The essential oil of Pistacia integerrima was found to be effective in protecting against oxidative renal damage. The protection is mediated by preventing the decline of renal antioxidant status. Conclusion: The present investigation demonstrates that the essential oil of Pistacia integerrima Stewart ex Brandis has a protective effect on cisplatin-induced experimental nephrotoxicity, and this effect is attributed to its direct anti-inflammatory and strong antioxidant profile. Hence, the essential oil of Pistacia integerrima Stewart ex Brandis has a solid potential to be used as a therapeutic adjuvant in cisplatin nephrotoxicity.

Keyword: Pistacia integerrima, essential oil, Cisplatin, Nephrotoxicity