Article: IN VITRO STUDY TO EVALUATE THE WOUND HEALING EFFECT OF HAMSAPADI (ADIANTUM LUNULATUM BURM.F.) KALKA IN FIBROBLASTS AND MUSCLE CELL LINES

Article Category: Original Research articles

DOI: 10.7897/2277-4343.166212

Pages: 49-53

Author: Mahendra Vaishnavi *, Sahana Kamath, Vishwanatha

Abstract: Ulcer is one of the most common ailments that affect mankind. Acharya Sushruta has explained 60 different types of therapeutic measures which are used to treat any ulcer and one among them was Kalka (paste). Hamsapadi (Adiantum lunulatum Burm. f.) was selected as Vrana ropaka (wound healing). The cytotoxicity of Hamsapadi Kalka (paste) on HEK293, McCoy and L6 cell lines were carried out using cell lines.Further the wound healing of McCoy and L6 cell lines were carried out to check the potential of wound healing of Hamsapadi Kalka(paste).In between 3 cell lines, overall Hamsapadi Kalka (paste) showed more cytotoxic to HEK293 cell line, moderate to McCoy and less toxic to L6 cell line. Muscle (L6) cell line showed more wound healing rate compared to Fibroblast (McCoy) cell line with different doses of Hamsapadi Kalka (paste). Cytotoxic and wound healing activity of Hamsapadi showed less toxic to muscle cell line (L6). Thus, it is an external treatment found to be an efficient Vrana ropaka (wound healing). This can be one of the natural, sustainable non-invasive measures for wound healing, especially in deeper tissues.

Keyword: Hamsapadi, wound, vrana (wound), kalka (paste).