Article: SCREENING OF CHAKRAMARDA (CASSIA TORA L.) PATRA FOR ITS ANTI-CANCER ACTIVITY ON SKIN CANCER CELL LINE: AN IN VITRO STUDY

Article Category: Original Research articles

DOI: 10.7897/2277-4343.17252

Pages: 64-66

Author: Narayanam Sai Sandeep *, Anuradha KN, Vishwanatha U

Abstract: Background: Chakramarda (Cassia tora L.) is an annual herb with various medicinal properties and indications in twak vikaras (skin disorders) such as dadru, pama, switra, and others mentioned in Ayurveda samhitha and nighantu. Skin cancer is one of the most prevalent types of cancer. The purpose of this study is to evaluate the efficacy of Chakramarda Patra (leaves) in vitro against skin cancer. Chakramarda is a well-known drug for twak vikara (skin disorders), according to ayurvedic classics. Objective: To evaluate and compare the anticancer effects of aqueous and hydro-alcoholic extracts of Chakramarda (Cassia tora L.) patra on skin cancer using an in vitro method. Methodology: The anticancer activity of aqueous and hydro-alcoholic extracts of Chakramarda (Cassia tora L.) Patra was evaluated in vitro using a squamous cell skin cancer cell line through a colorimetric cell viability assay, which was used for the evaluation of anti-cancerous activity. Results: A significant increase in the percentage of cell death was observed with increasing concentrations of both aqueous and hydro-alcoholic extracts. At a concentration of 1000 micrograms per milliliter, the aqueous extract showed 24.694 percent cell viability, while the hydro-alcoholic extract showed 8.600 percent cell viability. The standard anticancer drug cisplatin exhibited 1.583 percent cell viability. Conclusion: Both aqueous and hydro-alcoholic extracts of Chakramarda (Cassia tora L.) patra demonstrated significant anticancer activity against skin cancer under in vitro conditions. The hydro-alcoholic extract exhibited superior anticancer activity when compared to the aqueous extract.

Keyword: Anticancer activity, Kustaghna, Chakramarda, Cassia tora L., MTT assay, skin cancer cell line.