Article: COMPARATIVE ANTIMICROBIAL STUDY OF DIFFERENT EXTRACTS OF BARLERIA LUPULINA LINDL. AS A POTENTIAL SUBSTITUTE OF BARLERIA PRIONITIS LINN. (KATSAIREYA) IN INFECTIOUS DISEASES

Article Category: Original Research articles

DOI: 10.7897/2277-4343.17261

Pages: 118-125

Author: Ashwarya *, Rosy Gupta, Shiwani Sharma

Abstract: Katsaireya (Barleria prionitis Linn.) is mentioned in Ayurvedic classics for Krimighana (Antimicrobial), Vranaropana (wound healing), and Shothahara (Anti-inflammatory) indicating its antimicrobial, anti-inflammatory and wound healing properties. Another extrapharmacoepial Barleria species – Barleria lupulina Lindl. having similar phytochemical properties is compared with Barleria prionitis for antimicrobial action in the present study. Aqueous, ethanolic, and methanolic extracts of both the samples were tested against Escherichia coli (Gram Negative) and Staphylococcus aureus (Gram-Positive) bacteria using Kirby–Bauer Disc Diffusion and Agar Well Diffusion methods. Barleria lupulina exhibited greater antimicrobial activity than Barleria prionitis, with the aqueous extract showing the highest inhibition zone (22 mm) against Staphylococcus aureus and the methanolic extract significantly inhibited Escherichia coli. Antimicrobial activity increased with higher concentrations, and the Agar Well Diffusion method was found to be more effective than the Disc Diffusion method. The study concludes that Barleria lupulina possesses stronger antibacterial potential and may serve as an effective substitute for Barleria prionitis in Ayurvedic formulations targeting microbial infections.

Keyword: Barleria prionitis, Barleria lupulina, Escherichia coli, Staphylococcus aureus, antimicrobial activity, Krimighana, Vranaropana