Article: ANALYTICAL VALIDATION OF HRASHVA PANCHMOOLA TAILA: INTEGRATING TRADITIONAL FORMULATION PRINCIPLES WITH MODERN QUALITY ASSESSMENT

Article Category: Original Research articles

DOI: 10.7897/2277-4343.17262

Pages: 126-130

Author: Manju Nemiwal *, Harish Kumar Singhal

Abstract: Background: Hrashva Panchmoola Taila is a traditional Ayurvedic polyherbal compound described in the Ayurvedic text Charaka Samhita and mentioned in Vata Vyadhi Vikara. Given its therapeutic efficacy and rising clinical relevance, pharmacognostic and analytical quality standards have to be established in fulfillment of AYUSH and API recommendations. Aims and Objectives: To evaluate the pharmacognostic and physicochemical aspects of Hrashva Panchmoola Taila and create a chromatographic fingerprint for quality standardization. Materials and Methods: Hrashva Panchmoola Taila formulation contains medicinal compounds such as Laghupanchmoola, a collection of five roots widely used in Ayurveda for Vata-shamaka, Shothahara (anti-inflammatory), Vedanasthapana (analgesic), and tila pinyaka for Balya (strength-promoting) characteristics. Laghu Panchmoola and tila pinyaka are commonly used in inflammatory disorders, neuromuscular ailments, and pediatric conditions where Vata Dosha predominates. The above formulations were assessed using Thin Layer Chromatography (TLC), organoleptic characteristics and physiochemical properties. Results: Organoleptic testing indicated brick red formulation with a characteristic odor. Physicochemical investigation revealed that rancidity was absent, the saponification value was 199.10, the iodine value was 115.31, the refractive index was 1.4720, the acid value was 2.22, and the moisture content was 0.12%. TLC analysis has Rf values of 0.05, 0.10, 0.31, 0.40, 0.56, 0.75, 0.87, and 0.96 at 365 nm, showing the presence of several phyto-constituents. Conclusion: The current study establishes pharmacognostic, physicochemical, and chromatographic standards for Hrashva Panchmoola Taila. These parameters may be used as reference quality control standards to ensure the formulation's identity, purity, and consistency in line with AYUSH and API regulations.

Keyword: Analytical Validation, Thin Layer Chromatography, Quality Control, Drug Standardization.